Blood and Wound Culture, Lab Report Example

Unknown # 2 Wound Culture

Staphylococcus lugdunenesis is a staphylococcus strain which is gram negative. The S.lugdunensis infection can vary from a non-invasive skin commensal to an invasive pathogenic infection. The Staphylococcus lugdunensis disease can be the causal attribute of serious diseases that are similar to the virulent infections caused by Staphylococcus aureus. The consensus of the Staphylococcuslugdunensis bacteria can be treated with a substantial number of antimicrobial interventions.  The Staphylococcus lugdunensis strain was initially identified in 1988. It was differentiated from eleven other strains of Staphylococccus by the genetic studies which were founded on the known strains. The staphylococcus strain was designated after the name of the city of Lyons. This city is where the organism was first classified.  The unique quality that the Staphylococcus lugdunensis has is that it is the causal attribute of valve infective endocarditis. (Chu, 2014).

Procedure

The patient is a thirty five year old female who has the symptoms of a profound laceration on the elbow. The test was conducted by the use of the blood agar plate, the Mac Conkey plate, the CAN plate and the chocolate plate. The BB TSA agar 11 plate demonstrated that the colonies were gram positive and that there were whitecolo0nies. The colonies that were viewed were tiny and non- hemolytic.

Mac Conkey Plate

The Mac Conkey plate demonstrated that there had been no development and non- hemolysis. The colonies were a pink color. The colonies tested gram positive on the Mac Conkey plate.

CAN plate

The colonies were examined and tested non hemolytic. There was evidence of white colonies growing on the plate.

Chocolate Plate

There was evidence of tiny colonies that had a white color. The biochemical examination was subsequently performed. The PYR tested positive and there was a purple color that asserted the positive presence of ornithine.

Discussion

Staphylococcus lugdenensis is a disease which can rarely have the outcome of nativevalvic endocarditis. This strain is the causal attribute to light skin infections which have a similarity to the Staphylococcus aureus infections. A study was initiated in order to improve the methods of identification which are conducted in the laboratory environment. This laboratory study was ensued by a population based study of patients who9 had contracted the Staphylococcus lugdenesis in Viborg, Denmark. .The acknowledgement of this strain included the perception of an odor which was similar to the EikenellaCorrodens on the blood agar dish that was combined with the pleomorphic qualities of the colony that was being studied. .The prevalence of the ?- hemolysis became manifest subsequent to a two day period of incubation. The improved methods led to a 400% increase in the number of staphylococcus lugdenensisidentifications in the study which was conducted in Viborg, Denmark (Böcher 946).

Unknown #4 Blood culture

Klebsiella pneumonia pertains to the Klebsiella category of enterobacteriaca. This classification of bacteria can be found in the conventional flora of the human oral cavity and the intestinal tract. The Klebsiella pneumoniae is the most frequently encountered infection. The infections which consist of Klebsiella pneumonia are usually nocosomial and affect patients who have immune system deficiencies.

Procedure

The patient is an eighty nine year old male. The laboratory tests which will be applied are the blood agar plate and the Mac Conkey plate. The TSA plate demonstrated small white colonies. There was no hemolytic development and the colonies were minute in the quality.

 Mac Conkey Plate

There was growth and the colony was assessed to be gram negative. There was no pink color. This could be attributed to the inability of being able to decompose the lactose. A biochemical examination was performed. The oxidas test returned negative.  Consequently, an API test was required in order to determine the identity of the colonies. The API20E test returned with a zero result. The results of the API test are demonstrated on Table 1.

Table 1

Discussion

The elevated indexes of Klebsiella pneumonia transport in the nasopharyngeal area has been noted as attributed to patients who demonstrated symptoms of alcoholism. The rates of transport are increased in patients who have had previous tenures of hospitalization. The documented incidences are forty two percent in the area of the hands, nineteen percent in the nasopharyngeal area and seventy seven percent in the patients’ stool samples. The more elevated indexes of colonization are attributed to the consumption of antibiotics. The indexes of Klebsiellal transport have been proven to be four hundred percent more elevated in the patients who were carriers of the Klebsiella pneumoniae bacteria in comparison with those who were demonstrated to be non-carriers (Yu 1).

Works Cited

Böcher, Sidsel, BirgitteTemming and Robert Skov et al. “Staphylococcus lugdenensis.”A common cause of skin and soft tissue infections in the community. Journal of Clinical Microbiology 47.4(2009): 946- 950.

Chu, Vivian. “Staphylococcuuslugdensis.”Up to Date, 2014. Web. 15 September 2014. http://www.uptodate.com/contents/staphylococcus lugdensis

Hardy Diagnostics https://catalog.hardydiagnostics.com/cp_prod/content/hugo/refphoto/strepproFi   gure1.jpg

Flow Chart http://loudoun.nvcc.edu/vetonline/vet132/micro/flowchartGposcocci2.gif

Yu, Wen- Liang and Yin- Ching Chuang. Clinical features, diagnosis, and treatment of Klebsiella pneumonia infection.” Up to Date, 2014. Web.  15 September 2014. http://www.uptodate.com/contents/clinical-featyures-diagnosis-and-treatmnt-of-kklebsiella-pneumoniae-infection